Week 12

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In microbiology lab this week we completed an enzyme-linked immunosorbent assay (ELISA) test. This test detects antibodies in your blood to determine if you have been exposed to a disease. 

To begin we used a micropipette to add purified disease antigen to the correctly labeled wells of our microplate strip.

 We then washed the unbound antigen out of the wells and transferred the positive and negative control and the serum samples to the correctly labeled wells. 

After washing the samples out of the wells, we transferred the secondary antibody into all of the microplate wells. After waiting five minutes, we washed the unbound secondary antibody out and added an enzyme substrate into all twelve of the wells, recording our final results. 

This week in lab we also did an exercise on an antibody-antigen reaction in agar.  The purpose of this exercise was to determine if our sample hamburger extract was pure by observing a precipitin line.  First we made four wells within the agar and placed a different solution within each well. The solutions were: Bovine Albumin, Goat Anti-horse Albumin, Goat Anti-bovine Albumin, and Goat Anti-swine Albumin.  If the solution was pure there should have been a precipitin line observed between the Bovine Albumen and the Goat Anti-bovine Albumin. Unfortunately, due to the age of our solutions there was no precipitin line observed in our agar.