11/ 12/13
Today for lab we tested several different household disinfectants on a bacterial cultures to see their effects and the strengths and weaknesses of the different varieties of disinfectants. First we took a sample from a doorknob and spread it over a nutrient agar plate for culturing. Then we divided our plate into four sections and placed samples of four different, common household disinfectants. We used hand sanitizer (60% ethanol solution), toothpaste, Clorox, and mouthwash.
We then incubated our plates at 25 degrees Celsius.
Once we were finished we made another nutrient agar spread, but this time with our unknown bacteria. We all placed our samples in an anaerobic chamber to determine if our bacteria had anaerobic properties (placing the chamber in the incubator set at 37 degree Celsius)
11/14/13
The following class we observed our disinfectant testing, and found that our bacteria was unaffected by both hand sanitizer and mouthwash (Listerine).
Then we observed the results from the anaerobic test, and our bacteria did, in fact, have some growth (top sample). Thus showing that our bacteria is a facultative anaerobe.
Biochemistry ncubator lab action is to test the efficiency of numerous household housework crops or soaps for their sterile ability. Any non-pathogenic strain of a shared microorganisms, such as E. Coli can be used.
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