Week 7

Week 7

10/10/13

This week we observed the results from all of the tests we prepared last week.

We determined that our Starch hydrolysis test was negative, thus our bacteria does not have the enzymes necessary to digest starch.

Our casein hydrolysis test (skim milk plate) also was negative.

Our gelatin test was negative 

 

 Our fat (triglyceride) hydrolysis test was negative (our bacterial sample is on the bottom)

 

And finally, our Litmus milk test results:

The white curd at the bottom detects peptonization with an alkaline reaction. Thus, our bacteria has proteolytic enzymes that digest the casein (curd) into peptides and amino acids.

We were then instructed to inoculate test tubes filled with four different mediums to test if our bacteria had enzymes to digest carbohydrates (these test tubes were filled with lactose, sucrose, glucose, and Triple Sugar Iron (TSI) mediums).