week 1

In week one of lab, we first learned aseptic technique. We then performed an experiment to determine how much bacteria one has on his hands before and after hand washing. Each pair of students were given an agar plate and touched the agar with a finger (once before washing ones hands and once after). The agar plate was then put in an incubator so that whatever bacteria that was on the finger, before and after, would remain at optimal temperature for growth. Our initial hypothesis was that the finger print from before the hand washing would have cultured more bacteria than the finger print after, thereby confirming the need for proper hygiene; however, our results were the opposite.
        In the next lab, to our surprise, the majority of the class experiment had developed more bacteria in the  'post-hand washing'specimen then in the specimen taken before. We concluded that one of two things may have occurred: a) we did not practice good aseptic technique; or b) something we touched after washing our hands had a large quantity of bacteria, whether it was the paper towels we used to dry our hands or turning off the faucet after rinsing our hands.
       We also made a field trip to the beautiful steubenville marina, where we collected specimen from a nearby porter john and transferred it to an agar plate for culturing. We used a sterile broth solution (which we prepared in the previous lab session) to wet the swab and collect the specimen. Upon returning to campus, we placed our specimen into incubators so that the bacteria would grow at optimum temperatures.

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